MiR-125b-5p enclosed in hypoxic HK2 cell-derived extracellular vesicles alleviates renal ischemia-reperfusion injury by regulating NLRC5

Introduction In this study, we validated the changes in microRNA (miRNA) expression hypoxic human kidney 2 (HK2) cell-derived extracellular vesicles (EVs). Additionally, investigated mechanism by which miRNA that are derived from EVs alleviated renal ischemia-reperfusion (I/R) injury. Material and methods HK2 cells were treated a chamber (1% O2) for 12 h.EVs obtained as supernatant ultracentrifugation characterized. After examining 16 differentially expressed EV-miRNAs between normoxic conditions RT-PCR bioinformatics analysis, miR-125b-5p was selected further analysis. Normoxic cells-derived well isolated negative control (miR-NC)-transfected or mimic (miR-MI)-transfected injected mice with I/R The degree of injury assessed periodic acid-Schiff staining, tubule score, plasma creatinine levels. Bioinformatics analysis performed to determine potential target genes miRNAs. RT-PCR,western blotting, luciferase reporter assay, immunohistochemistry investigate relationship NLR family CARD domain containing 5 (NLRC5). Results revealed miRNAs, four upregulated. Animal study showed overexpression predicted NLRC5 targeted miR-125b-5p. Moreover, also validated. Conclusions There several miRNAs upregulated (including miR-125b-5p) cells. Hypoxia induced alleviate IRI, can be attributed targeting NLRC5.